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KWC was funded by NSERC grant #402264-2011. Funding Statement http://dx.doi.org/10.13039/501100004203Ontario Institute for Cancer Research IA-026 to Stephanie E Hallows. http://dx.doi.org/10.13039/501100000038Natural Sciences and Engineering [http://www.selleckchem.com/products/Paclitaxel(Taxol).html this website] Research Council of Canada 402264-2011 to Ko W Currie. http://dx.doi.org/10.13039/501100006126The Hospital for Sick Children Restracomp to Shu Jun Zhu. The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication. Funding Information This paper was supported by the following grants: http://dx.doi.org/10.13039/501100004203Ontario Institute for Cancer Research IA-026 to Stephanie E Hallows, Bret J Pearson. http://dx.doi.org/10.13039/501100000038Natural Sciences and Engineering Research [http://www.selleckchem.com/products/bgj398-nvp-bgj398.html check details] Council of Canada 402264-2011 to Ko W Currie. http://dx.doi.org/10.13039/501100006126The Hospital for Sick Children Restracomp to Shu Jun Zhu. Additional information Competing interests The authors declare that no competing interests exist. Author contributions SJZ, Conception and design, Acquisition of data, Analysis and interpretation of data, Drafting or revising the article. SEH, Conception and design, Acquisition of data, Analysis and interpretation of data. KWC, Conception and design, Acquisition of data, Analysis and interpretation of data. CJX, Conception and design, Analysis and interpretation of data. BJP, Conception and design, Drafting or revising the article. Additional files Supplementary file 1. RNA-deep sequencing (RNAseq) from this study and previously published studies and analysis. Raw reads from RNAseq of fluorescence-activated cell sorting (FACS)-isolated X1 and X2 populations, lethally irradiated (60 Gray) whole worms (Irrad), and intact control worms (WTcontrols). Tab 2, transcripts identified using DESq as having enriched expression in the X1 population compared to irradiated animals, with adjusted p-value [http://en.wikipedia.org/wiki/Fluconazole Fluconazole] Click here to view.(15M, xlsx) Supplementary file 2. Established stem cell and progeny genes, and genes characterized in this study. Tab 1�C2, listing of 59 verified neoblast-specific genes (Known stem cell genes) and five established postmitotic progeny genes previously shown to be enriched in the X2 FACS gate (Known X2 epithelial progenitor genes) used in Figure 1, Figure 7, and Figure 7��figure supplement 1. Tab 3, annotation of the top 100 X2-enriched genes chosen for expression analysis and RNA interference (RNAi) screening in this study. Tab 4, a total of 66 transcripts were identified as WThighXlow, which are irradiation-sensitive transcripts with low expression in both the X1 and X2 irradiation-sensitive FACS gates. Tab 5, epithelial progenitor markers identified in this study.
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Latest revision as of 17:50, 29 March 2022

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