Effect of MWCNTs on the lung inflammatory response in mice after sensitization with HDM allergen

Taqman quantitative RT-PCR b-actin was used as a reference gene. The relative transcript levels in the treated sample compared to the control sample were determined as fold changes outcomes exhibiting mRNA expression of professional-IL-one in THP-1s uncovered to 100 g/mL MWCNTs with IL-four, IL-thirteen, or IL-four and IL-thirteen co-therapy. Values directly above bars indicate significant difference from LPS treatment alone. Values above connecting lines indicate significant difference compared to MWCNT treatment. Data is pooled from three replicate experiments. (C) Representative Western blots showing protein levels of pro-IL-1, pro-caspase-1, phosphorylated (p)-STAT-6, STAT-6, and -actin after 24 hour exposure of LPS-primed THP-1 cells to MWCNTs in the absence or presence of IL-4, IL-13, or a combination of IL-4 and IL-13. (D) Quantitative densitometric analysis of pro-IL-1 Western blot signal from two replicate experiments. (E) Quantitative densitometric analysis of pro-caspase-1 Western blot signal from three replicate experiments. Statistical analysis performed using an unpaired student t-test. P