Effect of MWCNTs on the lung inflammatory response in mice after sensitization with HDM allergen

Taqman quantitative RT-PCR benefits exhibiting mRNA expression of pro-IL-1 in Clinical observations in humans suggest that an increased incidence of bacterial meningitis is associated with exposure to second hand tobacco smoke containing nicotine THP-1s exposed to one hundred g/mL MWCNTs with IL-four, IL-13, or IL-4 and IL-13 co-treatment method. Values above connecting lines indicate significant difference compared to MWCNT treatment. Data is pooled from three replicate experiments. (C) Representative Western blots showing protein levels of pro-IL-1, pro-caspase-1, phosphorylated (p)-STAT-6, STAT-6, and -actin after 24 hour exposure of LPS-primed THP-1 cells to MWCNTs in the absence or presence of IL-4, IL-13, or a combination of IL-4 and IL-13. (D) Quantitative densitometric analysis of pro-IL-1 Western blot signal from two replicate experiments. (E) Quantitative densitometric analysis of pro-caspase-1 Western blot signal from three replicate experiments. Statistical analysis performed using an unpaired student t-test. P