In order to monitor the spatio-temporal localization of Aurora A, whole mount immunofluorescence was performed on oocytes at different stages of in vitro maturation

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Punctate expression of Aurora A was detected in oocytes of all investigated stages from GV until finally MII (Fig five). In a tiny proportion (.05) in contrast to the amounts in handle oocytes (S1 Fig.).The expression of TACC3 was also directly knocked-down by means of injection of siRNA directed against TACC3 into GV stage oocytes. After injection the oocytes had been cultured for 23 h right after which they have been fixed and immunostained for TACC3 and tubulin expression. After injection of TACC3-siRNA the oocytes failed to experienced correctly. Much less (22.7%, n = 22) oocytes succeeded in extrusion of the 1st polar human body Stanniocalcin constitutes a small family of secreted homodimeric glycoproteins first found in the corpuscles of Stannius and has been implicated functional in the physiology following TACC3-siRNA injection in contrast with TRITC-injected (42.nine%, n = 28) and non-injected (sixty eight.four%, n = 117) oocytes.

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