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Results of these very first check details sets of experiments conducted to experimentally verify this possibility are described and discussed in this article. Possible implications of these findings for discovering drug leads from andrographolide and other structurally or functionally analogous secondary plant metabolites are also pointed out. 2.?Materials and methods 2.1. Animals Male and female Swiss albino mice (20?��?5?g) were acquired from the Central Animal House of the Institute of Medical Sciences, Banaras Hindu University, Varanasi, India (Registration Number: 542/AB/CPCSEA). Prior approval was granted by the Central Animal Ethical Committee of the Banaras Hindu University, Varanasi, India for the study protocol (Dean/11-12/CAEC/325, dated November 30, 2011), and the ��Principles of laboratory care�� (NIH publication number 85-23, revised during 1985) guidelines were always followed. The animals were housed in groups of six in polypropylene cages, and maintained at an ambient temperature of 25?��?1��C and 45�C55% relative humidity, with a 12:12 hours of light/dark cycle. They were provided with commercial food pellets and tap water ad libitum, and acclimatized to laboratory conditions for at least 1 week prior to using them in the experiment. All test parameters were assessed between 9.00 hours and 14.00 hours. 2.2. Drugs and chemicals Vorinostat datasheet Analytically pure andrographolide [99.0% by high-performance liquid chromatography GPX5 (HPLC); Fig.?1] isolated from A.?paniculata was generously supplied by R&D Center, Natural Remedies Pvt. Ltd., Bangalore, India. Details of the extraction procedure and analytical method used for standardization of the A.?paniculata extract used in our earlier studies have been reported elsewhere.23 Briefly, extraction of coarsely ground A.?paniculata leaves was performed with methanol for 3 hours, in a stainless�Csteel-jacketed extractor fitted with a reflux condenser. The liquid extract was removed, and the remaining raw material was re-extracted two more times with methanol in a similar manner. The resulting extracts were combined, concentrated, and dried under vacuum (at 30.0%, w/w), isoandrographolide (> 0.3%, w/w), neoandrographolide (> 1.0%, w/w), andrograpanin (> 0.3%, w/w), and 14-deoxy-11,12 didehydroandrographolide (