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These analytes were evaluated singly and in combination in a set of control and GDM maternal serum samples from the cohort described in Table 1. The mean participant age and pre-pregnancy BMI were 24.4 �� 3.5 years and 20.3 �� 3.3 kg/m2, respectively. Glycated hemoglobin measures did not differ between non-diabetic (5.2%; IQR: 5.0-5.4%) and GDM participants (5.4%; IQR: 5.1-5.8%). Fasting plasma glucose measures were also similar between groups 80 mg/dl (IQR: 77-85 mg/dl) and 84 mg/dl (IQR: 79-90 mg/dl); P = 0.20]. In addition, no statistically significant difference was observed between study groups for any other clinical parameter that was measured. Although [https://en.wikipedia.org/wiki/Fossariinae Fossariinae] fasting plasma glucose and glycated HbA1c measures were not different between groups, the levels of PSG-AAL, fibronectin-SNA [http://www.selleckchem.com/products/kpt-330.html click here] and the hCG/placental lactogen ratio were significantly elevated in the GDM group (P = 0.004, P = 0.006 and P = 0.03, respectively), as shown in Table 2. The difference in maternal serum CRP levels demonstrated borderline significance (P = 0.05), with a median concentration of 2.1 mg/l in non-diabetics and 5.7 mg/l in GDM participants. Placental lactogen and hCG exhibited altered levels in GDM maternal serum in previous studies.[26,27] These data suggest that combining these proteins in a ratio may improve discrimination ability. ROC curves utilizing fibronectin-SNA and PSG-AAL and the combination of these two analytes are shown in Figure 2. While the ability to detect GDM using both analytes is good (AUROC: 0.85), their use in conjunction with the [http://www.selleckchem.com/rac.html selleck] other analytes described Table 2 within a multi-analyte model [Figure 3] demonstrated clearly superior performance (AUROC: 0.97). Specifically, the combination of fibronectin-SNA and PSG-AAL alone had a detection rate of 74% at a false positive rate of 6%, while the multi-analyte model had a marked increase in the detection rate (87%) at a false positive rate
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Revision as of 16:24, 17 October 2016

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