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- 02:23, 20 December 2016 diff hist +27 m The elution was repeated once, and the fractions were pooled. DTT was added to 5 mM, and the mixture was incubated at 37uC for 45 min Tag: Reverted
- 02:22, 20 December 2016 diff hist +3,552 N The elution was repeated once, and the fractions were pooled. DTT was added to 5 mM, and the mixture was incubated at 37uC for 45 min Created page with "The soluble stage was centrifuged once more for twenty five min at 16,000 g at 4uC. Two hundred ml GammaBind Plus Sepharose beads (GE Health care) had been washed 3 times in P..."