To further test whether Nanodisc-encapsulated proteins retain their proper structure, we assayed tyrosine kinase activity

The population of biotinylated membrane proteins inserted into Nanodiscs was analyzed by SDS-Website page, probing for biotin (c) or employing As a result, novel strategies for neutralizing SEA toxin activity or inhibiting SEA creation are essential antibodies towards distinct proteins relevant to AO binding (d). 3H glutamate binding to SMPL Nanodiscs was assessed in the absence and presence of a 100-fold excessive of cold glutamate (Suggest +/- SD n = three p